State of Human Growth Hormone in Plasma and Changes in Stored Solutions of Pituitary Growth Hormone
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چکیده
منابع مشابه
State of human growth hormone in plasma and changes in stored solutions of pituitary growth hormone.
Radioimmunoassay of endogenous plasma growth hormone in human plasma fractions separated by starch gel electrophoresis shows that the plasma hormone migrates in a fairly narrow peak behind serum albumin. A similar electrophoretic mobility is exhibited by certain pituitary growth hormone preparations (the A type of Wilhehni), added to plasma in physiological concentrations or on electrophoresis ...
متن کاملImmunochemical investigations of human pituitary growth hormone.
Earlier immunological investigations of growth hormone (somatotropin) isolated from bovine and human pituitaries showed that these two protein hormone preparations form speciesspecific antibodies, as evidenced by the results of precipitin ring tests with rabbit antiserum and of anaphylactic shock experiments in guinea pigs (1, 2). It was further demonstrated that the rabbit antiserum exerts in ...
متن کاملRT-PCR MEDIATED CLONING OF HUMAN GROWTH HORMONE GENE AND I TS EXPRESSION IN E. coli
Human growth hormone (hGH) genomic sequence containing 5 exons and 4 introns was cloned in pcDNA-3 and the constructed plasmid was subsequently used for transfection ofNlli-3T3 cell line using lipofection technique. Expression of hGH in stably transfected cells was assayed using ELISA. Total RNA was extracted from transfected cells and hGH cDNA was amplified by RT-PCR using specific primers...
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BACKGROUND Mammographic density is a strong risk factor for breast cancer that is heritable and associated with blood levels of growth hormone and insulin-like growth factor-I (IGF-I). We tested single nucleotide polymorphisms (SNP) in pituitary growth hormone (GH1) and growth hormone-releasing hormone receptor (GHRHR) genes for an association with mammographic density, hormones of the growth h...
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Growth hormone (GH) is an essential element for normal growth. However, reports of normal growth without GH have been made in patients who have undergone brain surgery for craniopharyngioma. Normal growth without GH can be explained by hyperinsulinemia, hyperprolactinemia, elevated leptin levels, and GH variants; however, its exact mechanism has not been elucidated yet. We diagnosed a female pa...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1966
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)96335-0